Friday, May 20, 2022
No Result
View All Result
Medical Finance
  • Home
  • News
  • Interviews
  • Mediknowledge
  • Insights From Industry
  • Thought Leaders
  • Coronavirus
  • Whitepapers
  • Home
  • News
  • Interviews
  • Mediknowledge
  • Insights From Industry
  • Thought Leaders
  • Coronavirus
  • Whitepapers
No Result
View All Result
Medical Finance
No Result
View All Result
Home Coronavirus

Substantial SARS-CoV-2 infection in perivascular cells of inflamed blood vessels

by Medical Finance
in Coronavirus
Study: Delineating antibody escape from Omicron variants. Image Credit:  Dkoi/Shutterstock
9
SHARES
100
VIEWS
Share on FacebookShare on Twitter

A recent article posted to the bioRxiv* preprint server demonstrated that vascular inflammation leads to heightened severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in perivascular cells.

Study: Delineating antibody escape from Omicron variants. Image Credit:  Dkoi/Shutterstock
Study: Delineating antibody escape from Omicron variants. Image Credit: Dkoi/Shutterstock

Background

Blood vessels are composed of endothelial and perivascular cells. Pericytes are crucial for stabilizing blood vessels and the functioning of the vascular barrier. Pericytes and endothelial cells (ECs) harbor angiotensin-converting enzyme 2 (ACE2) receptor, the primary host interaction site of the SARS-CoV-2 spike 1 (S1) protein. 

Numerous studies depicted the vascular consequences of SARS-CoV-2 infection and the role of pericytes in disease progression. Yet, the relevance of perivascular cells and inflammation in coronavirus disease 2019 (COVID-19) is unknown. Moreover, prior reports state a higher preference of SARS-CoV-2 for pericyte-binding than ECs in some organs.

However, it is unclear whether a previous vascular barrier disruption influences the binding of the SARS-CoV-2 particles to perivascular cells or ECs or not.

About the study 

In the present work, the researchers hypothesized that in healthy vascular capillaries with an intact barrier function, SARS-CoV-2 has limited entry to perivascular cells, which results in controlled immune stimulation and minimal vascular damage. On the other hand, blood vessels with a compromised barrier function have an elevated rate of SARS-CoV-2 extravasation. Additionally, blood vessels with pro-inflammatory mediators such as tumor necrosis factor α (TNFα) present an altered functioning barrier. 

Subsequently, the team tested the hypothesis by employing two well-known vascular capillary on-chip models. The authors improved the models by constructing endothelial capillaries backed with pericytes/perivascular cells derived from the mesenchymal stromal cells (MSC). The researchers assessed the impact of vascular inflammation on the selective adherence of the SARS-CoV-2 S1 protein to perivascular cells employing the models.

In the central chamber of the microfluidic device used to engineer the microphysiologic model of pericyte-supported microvasculature on-chip, the researchers planted a cell suspension in a collagen-fibrin hydrogel comprising encased human MSCs (hMSCs) and green fluorescent protein (GFP)-expressing human umbilical vein ECs (GFP-HUVECs), in a 4:1 HUVEC:hMSC ratio. 

Results and discussions

The study results illustrated that the SARS-CoV-2 S1 protein was linked with both TNFα treated and untreated/control capillaries, yet the binding was more significant in TNFα-treated ones. TNFα-treated capillaries had an even distribution of the S protein continually colocalized with cells inside the perivascular region’s extracellular matrix and the microvascular network. In contrast, control blood vessels had an uneven distribution of the S protein throughout the capillaries, with increased concentration at the junctions. In the perivascular area of control vessels, there were just a few S protein spots.

The control vessels demonstrated a thin endothelialized layer of ECs colocalized with perivascular cells showing a faint stain of the S protein attached to the vessel wall and in certain migrating cells inside the surrounding connective tissue. On the other hand, the S1 protein was mostly linked to delaminating perivascular cells in TNFα-treated samples. While no S1 proteins were observed outside the blood vessel in controls, TNFα treatment caused the loss of barrier function, allowing S1 proteins to pass through the endothelial barrier and remain outside the vessel.

The number of migrating perivascular cells was double in the cells exposed to TNFα relative to the controls. Furthermore, perivascular cells exposed to TNFα became large, developed migratory characteristics with stellate-shaped and elongated cytoplasm, with main activities parallel to the lengthy vascular axis, and many conspicuous filopodia directed outside the vessel, within the adjacent extracellular matrix.

Additionally, while the spatial correlation between S1 protein and GFP signal in controls was 0.9, TNFα-treated vessels exhibited a lower S1 protein and GFP spatial relationship, illustrating that the S1 protein distribution was substantially wider in inflamed vessels.

Conclusions

The study findings established a significant association between vascular function, inflammation, and perivascular cells. This association could impart a new understanding of the pathogenesis of SARS-CoV-2 infection and assist in justifying the extensive effects of the infection in several vascularized organs and tissues of the body.

The present study depicts a drastically higher adherence and extravasation of the SARS-CoV-2 S1 protein with perivascular cells of inflamed blood vessels than the healthy vessels. The S1 protein had increased access to perivascular domains with TNFα-facilitated inflammation. The study findings indicate that perivascular cells might be a possible target for addressing the worsened vascular consequences of people with inflammatory comorbidities during COVID-19.

*Important notice

bioRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.

Journal reference:

  • Henning Gruell, Kanika Vanshylla, Michael Korenkov, Pinkus Tober-Lau, Matthias Zehner, Friederike Muenn, Hanna Janicki, Max Augustin, Philipp Schommers, Leif Erik Sander, Florian Kurth, Christoph Kreer, Florian Klein. (2022). Delineating antibody escape from Omicron variants. bioRxiv. doi: https://doi.org/10.1101/2022.04.06.487257 https://www.biorxiv.org/content/10.1101/2022.04.06.487257v1
Total
0
Shares
Share 0
Tweet 0
Pin it 0
Share 0
Medical Finance

Medical Finance

Related Posts

Study: A method for variant agnostic detection of SARS-CoV-2, rapid monitoring of circulating variants, detection of mutations of biological significance, and early detection of emergent variants such as Omicron. Image Credit: Lightspring/Shutterstock

New method for monitoring circulating SARS-CoV-2 variants and detecting new ones

by Medical Finance
May 20, 2022
0

After more than two years of the coronavirus disease (COVID-19) pandemic, there is still a constant threat of evolving strains...

Providing recombinant antibody services and more within Europe

Providing recombinant antibody services and more within Europe

by Medical Finance
May 20, 2022
0

In this interview, News-Medical talks to Dr. Zhang Linlin, Technical Specialist at Sino Biological Europe, about their recombinant antibody services...

Study: Temporary hold of mycophenolate boosts SARS-CoV-2 vaccination-specific humoral and cellular immunity in kidney transplant recipients. Image Credit: PhotobyTawat/Shutterstock

Withdrawing steroid treatment found to boost SARS-CoV-2 vaccine-induced immunity in kidney transplant recipients

by Medical Finance
May 20, 2022
0

In a recent study posted to the medRxiv* pre-print server, a team of researchers investigated the impact of the fourth...

Study: Higher viral load and infectivity increase risk of aerosol transmission for Delta and Omicron variants of SARS-CoV-2. Image Credit: Tavarius/Shutterstock

Study reveals factors responsible for increased risk of aerosol transmission for SARS-CoV-2 Delta and Omicron

by Medical Finance
May 20, 2022
0

In the past, studies have indicated that the virus concentration in a room when infected humans exhale aerosols can be...

Fire closes hospital and displaces staff as Colorado battles omicron

Is my cloth mask good enough? The 2022 edition

by Medical Finance
May 20, 2022
0

The highly transmissible omicron variant is sweeping the U.S., causing a huge spike in covid-19 cases and overwhelming many hospital...

Study: Evaluating Real-World COVID-19 Vaccine Effectiveness Using a Test-Negative Case-Control Design. Image Credit: siam.pukkato/Shutterstock

Real-world effectiveness of three COVID-19 vaccines

by Medical Finance
May 20, 2022
0

The mass lockdowns and restrictions imposed by governments to prevent the spread of coronavirus disease 2019 (COVID-19) were dominant...

Next Post
Study: Engineered small extracellular vesicles displaying ACE2 variants on the surface protect against SARS-CoV-2 infection. Image Credit: NIAID

Engineered small extracellular vesicles for the neutralization of SARS-CoV-2

Influenza vaccination does not affect risk of SARS-CoV-2 infection or COVID-19

Influenza vaccination does not affect risk of SARS-CoV-2 infection or COVID-19

0 0 votes
Article Rating
Subscribe
Login
Notify of
guest
guest
0 Comments
Inline Feedbacks
View all comments

Support

  • Contact
  • Disclaimer
  • Home
  • Privacy Policy
  • Terms And Conditions

Categories

  • Coronavirus
  • Insights From Industry
  • Interviews
  • Mediknowledge
  • News
  • Thought Leaders
  • Whitepapers

More News

  • 163926142 620x480
    Researchers test how three mutations work together to produce new SARS-CoV-2 variants
  • Image Credit: MAD.vertise/Shutterstock.com
    Pediatric Robotic Surgery: past, present and potential
  • Home
  • Privacy Policy
  • Contact
  • Disclaimer
  • Terms And Conditions

© 2022 Medical Finance - Latest Financial and Business News

No Result
View All Result
  • Interviews
  • Mediknowledge
  • News
  • Insights From Industry
  • Coronavirus
  • Thought Leaders
  • Whitepapers
wpDiscuz
0
0
Would love your thoughts, please comment.x
()
x
| Reply